potential co-treatment effects of aataria multiflora, camellia sinensis extracts and bacterial lipopolysaccharide on skin wound model

Camellia sinensis and Zataria multiflora as the potent antioxidants and antiinflammations are crucial in the management of wound therapy. Lipopolysaccharide [LPS] was used for the proliferative potential on fibroblast cells and induction of inflammatory mediators

Objective: We examined the effects of LPS and herbal extracts combination in order to identify their mechanisms of action in fibroblast proliferation and tissue regeneration

Methods: Human foreskin fibroblasts were treated by Salmonella enterica LPS [100micro g] and extracts [5%w/w]. Tissues of male Balb/c mice were harvested at 1, 3 and 7 days for biochemical and histopathological evaluations. Effects of LPS and extracts on cell viability, Nitric oxide [NO], Cyclooxygenase-2 [COX-2] and Hydrogen peroxide H[2]O[2] levels were examined respectively by XTT assay and related kits

Results: Our pathological investigations for Camellia sinensis and LPS co-treated group indicated inflammatory cells on day 1 and fibroblast proliferation through wound area on day 3. After wound modelling the samples features were the same but with the difference in groups treated by LPS and Camellia sinensis extract which dermis and epidermis were seen. The Camellia sinensis extract and LPS co-treated wounds were showed low levels of H[2]O[2] and higher levels of NO compared to extract treated group [P<0.001]. Results illustrate a dose and time dependent significant difference in cell proliferation between groups

Conclusion: These results suggest that Camellia sinensis extract in combination with LPS may have potential of accelerating inflammatory phase of wound healing process by regulation of COX-2, NO and H[2]O[2] in skin fibroblast

Zingiber officinale protective effects on gentamicin's toxicity on sperm in rats

Ginger rhizome [Zingiber officinale R., family: Zingiberaceae] is used medicinally and as a culinary spice and has anti-oxidant and cell-protective effects in animals and humans body. The aim of this study was to investigate the influence of ginger rhizome toxicity of gentamicin on sperm parameters in male rats. Forty Wistar male rat [n=40] were allocated into four groups, control [n=10] and test groups [n=30], that subdivided into groups of 3 that received ginger rhizome powder [100 mg/kg/day], gentamicin group that received, 5 mg/kg/day and ginger group that received, 5 mg/kg/day gentamicin additionally, for 30 consequence day. Animals were kept in standard conditions. In thirty day the testes tissue of rats in whole groups were collected. Ginger administration caused a marked increase in the testosterone concentrations of the rats even in spite of receiving 5 mg/kg/day gentamicin in compared with the control and gentamicin treated groups. Ginger rhizome is able to overcome reproductive toxicity of gentamicin and induces spermatogenesis probably mainly through the elevation of testosterone levels

Effects of Danae racemosa on testosterone hormone in experimental diabetic rats

Danae racemosa is a strong antioxidant and antioxidants have significant effects on spermatogenesis, sperm biology and oxidative stress, and changes in antioxidant capacity are considered to be involved in the pathogenesis of chronic diabetes mellitus. Because STZ causes testicular dysfunction and degeneration under situations of experimentally induced diabetes in animal models, we aimed in this study Danae racemosa's effects in decreasing the harmful effects of STZ on testicular and sperm functions b. Male Wistar rats [n = 40] were allocated into four groups: Group 1a: Control rats given 0.5 ml of 20% glycerol in 0.9% normal saline. Group 1b: Control rats given 0.5ml of 0.5 ml citrate buffer [pH4.0].Group 2: streptozotocin [STZ] treated rats. Group 3: rats given danae racemosa 400 mg/kg [gavage]. Group 4: STZ treated rats given danae racemosa 400 mg/kg [gavage]. Animals were kept in standard conditions. At 28 days after inducing diabetics, 5 mL blood was collected for measuring testosterone. Total serum testosterone increased significantly in the group treated with danae racemosa [p < 0.05] compared with control groups. Testis weights in the diabetic groups decreased significantly in comparison with controls [p < 0.05]. Danae racemosa had a significant protective effect on the diabetes-induced deteriorations in serum total testosterone, by reducing the levels of reactive oxygen species in serum. Therefore, it could be effective for maintaining healthy in diabetic rats

Treatment effects of Ginger rhizome and extract of carrot seed on diabetic nephropathy in rat

Antioxidants have essential effect on tissue regeneration after cells injury. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Ginger rhizome and carrot seed are strong antioxidants and long-term treatment of Streptozotocin induced-diabetic animals with these herbs, has been shown to reduce oxidative stress. Evaluation to treatment effect of Ginger rhizome and extract of carrot seed on nephropathy after diabetes inducement. Methods: Wistar male rat [n=70] were allocated into seven groups, control group, carrot seed extract group, ginger group, control- Diabetic group received 55mg/kg [IP] streptozotocin [STZ], treatment diabetic group that received carrot seed extract, treatment diabetic group that received ginger and treatment diabetic group that received carrot seed extract plus ginger. Animals were kept in standard condition. In 30 day after inducing diabetes, 5ml blood were collected for analyzing of TAC and MDA levels, and kidney tissues of Rats were removed in all groups then prepared for analysis. Pathological changes in diabetic group which received carrot seed and ginger together was decreased compared to control group. The rate of serum TAC significantly increased in diabetic groups which received carrot seed and ginger together significantly in comparison to control-diabetic group [p<0.05]. Since in our study 25 mg/kg carrot seed extract and 100 mg/kg ginger have prevented kidney tissue injury by reducing level of Reactive Oxygen Species [ROS] in serum, so it seems that using it can be effective for treatment nephropathy in Diabetic rats

Evaluation of protective effects of nano-formulated silymarin against UV radiation on guinea pig skin

As increased outdoor activities of people, they are more exposed to UV radiation, which causes harmful effects on skin. Silymarin has protective potentials against DNA damage and non-melanoma skin cancers, and also it has anti-oxidant, anti-inflammatory, and immune regulation characteristics. Nano formulation of plant material showed to be more effective than common ones. In present study, nano-formulated silymarin was used in a cream, and its effects were investigated against destructive effects of UV rays. Methods: Silymarin standardized extract was prepared, nano formulated in cream base, and applied to skin of the animals. Four groups of animals [n=15] were examined; Group 1; without any treatment [control], Group 2; Vaseline, Group 3; nano-formulated silymarin extract cream, and Group 4; silymarin-carnosine. UV was radiated on the shaved area of the four groups for 45 minutes with dose of 180 mJ/cm2. Dermal scaling, skin irregularity, erythema, skin hyperpigmentation, and edema were evaluated in animals. Histopathological observation was done on the skin samples of the animals. silymarin nano-formulation was effective in decreasing both physical damages and histopathological injuries significantly. from the observed results, it can be concluded that the cream containing nanoformulated silymarin can prevent UV radiation lesions better than pure silymarin

Compartments of Quercetin and Allium cepa [onion] on blood glucose in diabetic rats

The major concern in diabetes is increased oxidative stress. Maintaining a balance between reactive oxygen species [ROS] and antioxidants is a major mechanism in preventing damage from oxidative stress. Quercetin [QR] is a well-known flavonoid and a strong antioxidant derived from the onion, Allium cepa, and it has been shown to reduce oxidative stress in the long-term treatment of streptozotocin [STZ]-induced diabetes in animals. We aimed to study on beneficial effects of QR and Allium cepa on regulation of blood glucose in diabetic model. Methods: Seventy male 8-week-old Wistar albino rats weighing 250 +/- 10 g were obtained, the rats were divided into seven groups comprising ten animals in each group. Diabetes induced with single intraperitoneal injection of STZ. Diabetic rats treated with quercetin [15mg/kg/day, started 48 hours after STZ injection] and Allium cepa juice [2cc/rat/day, started 48 hours after STZ injection]. After 28 days treatment, analysis on blood glucose and insulin was done. The mean TAC showed a significant increase [p < 0.05] in the QR compared to the control, STZ and STZ + QR groups. Both QR and onion Juice had significant effect in lowering blood glucose [355.3 +/- 104 mg/dl and 353.4 +/- 103 mg/dl respectively] [p<0.05] in STZ induced diabetic animals. Both QR and onion juice had lowering effect in Serum insulin in healthy animals and significant increasing effect on serum insulin level in diabetic animals. Both QR and onion juice had good effect in modifying diabetes related biochemical parameters and they are recommended to be studied more in order to being used in the therapy

[Treatment effect of onion on sexual behavior after induces an antiepileptic drug [lamotrigine] in male rat]

The medicinal use of onion dates back to ancient China and India. Men with epilepsy have reduced fertility, and antiepileptic drugs may affect semen quality. Disturbances of reproductive endocrine hormones are more often found in men with epilepsy than in the general population. There is an ongoing debate whether this can be attributed to chronic use of antiepileptic drugs or to the epilepsy itself. The aim of the present study was to evaluate the beneficial degree of sexual behavior in male rats after inducement onion in lamotrigine receiving groups. Forty wistar rats, randomized into four groups [n=10], were used for this study, five of them male and five of them female. Animals in Group A served as the control and was drinking distilled water. Animals in Groups B: treated with 3cc/rat of onion juice, in Groups C: received10mg/kg/day lamotrigine, in Groups D: received10mg/kg/day lamotrigine plus 3cc/rat of onion juice. In All treatments were for 5 weeks. 48 hours before end of study estradiol benzoate [25 micro gr/kg/day, s.c.] and 6 hours before end of study progesterone 0.3muM were injected. Results showed the percentage of erections and couplings and serum testosterone in onion treated groups were significantly increased [p<0.01] when compared to other groups. onion and its constituents are stated to has antioxidant. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis in diseases. These findings lead to the conclusion that onion significantly lowered the adverse effects of lamotrigine, and can do beneficial effect on sexual behavior in male rat

[Study of oral administrated silymarin effects on UV-induced changes on albino guinea pigs skin]

Studies have shown that Silymarin is one of the infrequent herbal materials which have protective effects against sunburn, non melanoma skin cancer and immunosupression in oral administration. Easiness of use, low cost and availability of Silymarin can propose it for use in many topical sunscreen products. Regarding to the skin damages caused by the ultraviolet ray of sun and necessity to use of protective agents against mal effects of this ray, in this study the effects of oral use of Silymarin against effects of UV irradiation was evaluated. In this study 60 albino guinea pigs were selected and they divided in two equal groups. Animals in the experimental group administered 50 mg/kg Silymarin 20 days before and through the irradiation period orally. Control group received distilled water only. Then each group exposed to 180mj/cm2 UV ray. Finally clinical signs were noted and sampling was done from irradiated skin areas for histopathological evaluation. Histopathological and clinical findings showed that oral administration of Silymarin had protective effects against mal effects of UV irradiation on guinea pig skins. The results of this study can be incentive for introduction and use of this herbal agent in sun ray protective oral products because any topical sunscreen has not perfect protective effects against all UV irradiation spectrums

[Increasing rate of human bone marrow mesenchymal stem cell proliferation in the presence of silymarin]

Mesenchymal stem cells [MSCs], the non-hematopoietic progenitor cells found in various adult tissues are characterized by their ease of isolation and their rapid growth in vitro while maintaining their differentiation potential, allowing for extensive culture expansion to obtain large quantities suitable for therapeutic application. Silymarin has strong antioxidant and anti-inflammatory activities with positive effect on proliferation on some cell types. The aim of this study was to find out the optimal condition of silymarin treatments on mesenchymal stem cell growth and multiplication. Human MSCs in third passage were divided into 12 groups treated by 50, 75, and 100 micro g/ml of silymarin for 2, 7 and 14 days. Cell viability was assayed on day 2 using trypan blue exclusion test. The cell proliferation rate in presence of silymarin was determined using a day-response curve for each dose. Viability was 89%, 93%, and 96% for cells treated with 50, 75, and l00 microg/ml silymarin, respectively. Cell viability showed significant increase in all treated cells in comparison with the control group [83%]. Based on the day- response curve, it was shown that the rate of cell proliferation in treated cells is significantly higher when exposed to silymarin for 2 - 7 days. However, from 7[th] day to 14[th], silymarin exposure lowers hBMSCs proliferation rate compared to control group. Under optimal condition of silymarin exposure time, the rate of MSC proliferation can be stimulated

[Study on phenolics and antioxidant activity of some selected plant of mazandaran province]

Plants are rich in phenolics [phenolic acids, flavonoids and Tannins]. The interest in phenolics has been increased by recent reports of their antioxidant activities. These antioxidant nutrients from food sources reduce the harmful effects of oxidative stress and offer health advantages. Total phenolics, flavonoids contents and antioxidant capacity of seven culinary plants of Mazandaran province were investigated and compared to Rosemary. Methanolic extracts of dried powdered aerial parts of plants were prepared. Total phenolic compounds in extracts were determined spectrophotometrically using the Folin- Ciocalteu reagent. Antioxidant activity assessed by using 2, 2-diphenyl-1-picrylhydrazyl [DPPH] method. The total phenolic content of plants extracts ranged from 38.27 to 58.45 mgGAEg[1] [Gallic acid equivalent/g dried weight], flavonoid content ranged from 25.5 to 182.23 mgQUEg[1] [mg Quercetin equivalent/g dried weight] and IC[50] ranges from 55.52 to 489.9 microgml[-1]. Among the 7 different plants, Marrubium vulgare contained a higher level of phenolics and high antioxidant capacity compared to Rosemary, while Mentha spicata had the lowest content of phenolics. The antioxidant capacity determined by the 2, 2-diphenyl-1-picrylhydrazyl [DPPH] method was lowest for of Mentha spicata. These results clearly showed the antioxidant activity could be correlated with the phenolic components content in the extracts

Study the effects of oral administration of silymarin in preventing consequences of ethanol on liver during pregnancy

Alcoholic mothers who consume ethanol give birth to infants with high percentage of hepatic diseases. Alcohol can cause cellular damage in different tissues, including liver. Finding a drug which is effective and efficient in reducing ethanol misuse consequences during pregnancy can assist the decrease of harmful effects of this habit. This research aims to study the effects of oral administration of silymarin in preventing consequences of ethanol on liver during pregnancy. 45 female rats were randomly divided into 3 groups, each including 15 ones. After the first day of pregnancy, the study was performed as follows. The first group was given distilled water. The second group received ethanol equivalent to 35% of the total required calorie. Furthermore, the third group received the same amount of ethanol plus 200 mg/kg silymarin. At day 21, biopsy of liver tissue of the born infants was carried out for light microscopy studies. Findings of the present study indicate that, in the group which received ethanol, this extract can have a considerable effect on decreasing the hepatic cellular death as well as reducing hepatic hyperemia. Oral consumption of silymarin extract can reduce the damage and inflammation in liver tissue of infants born from alcoholic mothers

[Evaluation of Ocimum basilicum effects on uterus apoptosis in rats exposures in electromagnetic fields]

In the industrial world, almost everyone is unavoidably exposed to ambient electromagnetic field [EMF] generated from various technical and household appliances. Controversy exists about the effects of EMF on various tissues of the living bodies. Uterus play an important role in reproductive and the effects of EMF on its tissue is worthy of investigation. In order to examine this, 40 rat were selected and kept for one weeks in quarantine and 20 rats subdivided to two groups were exposed to 50 Hz [non-ionizing radiation] for 6 weeks, one of this exposed group received 0.7 g/kg Ocimum basilicum, other experimental group just received 0.7 g/kg Ocimum basilicum, The materials were processed and observed apoptosis under a light microscope. In the experimental rats apoptotic cells showed significantly increased in emf groups, but in exposed group that received Ocimum basilicum, apoptotic cells were decreased [p<0.05]. As exposure to EMF may result in pathological changes then lead to sub fertility and infertility, Since in our study 0.7 g/kg Ocimum basilicum have significantly Preventive effect on uterus cells damages by reducing number of Apoptotic cells, so it seems that using it can be effective for treatment in emf exposed Rat

[Treatment effects of onion on spermatogenesis in streptozotocin-induced diabetic rat]

Onion has significant blood sugar lowering action. Antioxidants have essential effect on spermatogenesis and sperm parameters. Streptozotocin can dioceses oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat [n=30] were allocated into three groups, control group [n=10] and onion [O] group that received 1cc/rat [gavages]; [n=10], and Diabetic group that received 55mg/kg [IP] streptozotocin [STZ] [n=20] which was subdivided to two groups of 10; STZ group and treatment group. Treatment group received 55 mg/ kg [IP] STZ plus 15 mg/kg [O], daily for, 4 weeks, respectively; however, the control group just received an equal volume of distilled water daily[gavages]. Diabetes was induced by a single [IP] injection of streptozotocin [55mg/kg]. Animals were kept in standard condition. In 28 day after inducing diabetic 5cc blood were collected for testestrone, TAC, MDA and Ox-LDL levels and testes tissues of Rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis. Sperm population, percentage of sperm viability and motility and Serum total testosterones significantly increased in group that has received 1cc/rat onion [p<0.05] in significantly decreased in comparison to control group [p<0.05]. Since in our study 1cc/rat onion have significantly preventive effect on Sperm percentage of viability and motility and serum total testosterones by reducing level of Reactive Oxygen Species [ROS] in serum, so it seems that using it can be effective for sperm healthy parameters in Diabetic Rat

Effects of ginger on spermatogenesis in streptozotocin-induced diabetic rat

Ginger rhizome [Zingiber officinale R., family: Zingiberaceae] is used medicinally and as a culinary spice. The medicinal use of ginger dates back to ancient China and India. Ginger and its constituents are stated to have antiemetic, antithrombotic antihepatotoxic, anti-inflammatory, stimulant, cholagogue and antioxidant. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat [n=40] were allocated into three groups control group [n=10] and ginger [G] group that received l00mg/kg-perday [oral], [n=10], and Diabetic group that received 55 mg/kg [IP] streptozotocin [STZ] [n=20] which was subdivided to two groups of 10; STZ group and treatment group. Treatment group received 55mg/kg [IP] STZ plus ginger l00mg/kg-perday [G], daily for, 4 weeks, respectiveiy; however, the control group just received an equal volume of distilled water daily[IP]. Diabetes was induced by a single [IP] injection of streptozotocin C55 mg/kg]. Animals were kept in standard condition. In 28 day after inducing diabetic 5cc blood were collected for TAC, MDA and sperm parameters and testis tissues of Rat in whole groups were removed then prepared for analyzes. TAC, MDA and sperm parameters significantly decreased in diabetic group.treatment groub that has received Ginger 100 mg/kg [oral] show increasing in TAC and sperm parameters [p<0.05] in comparison to experimental groups [p<0.05]. Since in our study 100 mg/kg [oral] ginger have significantly Preventive effect on sperm parameters, so it seems that using it can be effective for treatment in Diabetic Rat

Protective effects of Quercetin on spermatogenesis in streptozotocin-induced diabetic rat

Quercetin is a strong antioxidant and long-term treatment of STZdiabetic animals and it has been shown to reduce oxidative stress. antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat [n=40] were allocated into three groups, control group [n=10] and Quercetin [QR] group that received 15mg/kg [IP] QR, [n=10], and Diabetic group that received 55 mg/kg [IP] streptozotocin [STZ] [n=20] which was subdivided to two groups of 10; STZ group and treatment group. Treatment group received 55 mg/kg [IP] STZ plus15mg/kg QR, daily for,4 weeks, respectively; however, the control group just received an equal volume of distilled water daily [IP]. Diabetes was induced by a single intra peritonea injection of streptozotocin [55 mg/kg]. Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for testosterone, TAC, MDA and Ox-LDL levels and testes tissues of Rat in whole groups were removed and sperm was collected from epididymis then prepared for analysis. Sperm population, percentage of sperm viability and motility significantly increased in group that has received 15 mg/kg [IP] Quercetin [P < 0.05] in comparison to control and experimental groups. Since in our study 15 mg/kg [IP] Quercetin have significantly Preventive effect on Sperm percentage of viability and motility by reducing level of Reactive Oxygen Species [ROS] in serum, so it seems that using it can be effective for sperm healthy parameters in Diabetic Rat

Evaluation effects of quercetin on liver apoptosis in streptozotocin-induced diabetic rat

Quercetin is a strong antioxidant and long-term treatment of STZdiabetic animals and it has been shown to reduce oxidative stress. Antioxidants have essential effect on spermatogenesis and sperm parameters. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Wistar male rat [n=40] were allocated into three groups, control group [n=10] and Quercetin [QR] group that received 15mg/kg [IP] QR, [n=10], and Diabetic group that received 55mg/kg [IP] streptozotocin [STZ] [n=20] which was subdivided to two groups of 10; STZ group and treatment group. Treatment group received 55mg/kg [IP] STZ plus15mg/kg QR, daily for,4 weeks, respectively; however, the control group just received an equal volume of distilled water daily[IP]. Diabetes was induced by a single [IP] injection of streptozotocin [55mg/kg]. Animals were kept in standard condition. In 28day after inducing diabetic 5cc blood were collected for TAC,MDA and Ox-LDL levels and Liver tissues of Rat in whole groups were removed then prepared for Apoptosis analysis by Tunel method. Apoptotic cells significantly decreased in group that has received 15mg/kg [IP] Quercetin [P < 0.05] in comparison to experimental groups [P < 0.05]. Since in our study 15mg/kg [IP] Quercetin have significantly Preventive effect on liver cells damages by reducing number of Apoptotic cells in liver, so it seems that using it can be effective for treatment in Diabetic Rat

[Study on effects of Cassia Angustifolia vahl. syrup on liver and kidney of rat by analysis of biochemical factors]

Cassia Angustifolia Extract [CA] has indication as a colon irritant. For rapid and full action of this extract or their derivatives need to high dosage of them. CA can have toxic effects on different organ of body, therefore it is necessary to study effects of them on liver and kidney. In this study effect of Cassia Angustifolia syrup on liver and kidney of rat were assigned by analysis of biochemical factors which related to these organs. 40 rats randomly divided in two groups with 20 members. First group was received 0.9 mi/kg CA containg of 1.85 mg CA extract in 30ml sterile water. Second group was received only 30ml sterile water. After 1, 24, 48 and 72 hours blood samples were taken from these groups and then analysis of biochemical factors related to liver and kidney activity were done. Also, the clinical conditions were recorded four times a day. diarrhea as a clinical condition showed only in the first group during 72 hours. 1 hour after receiving CA or water in both groups the ALP, ALT and AST showed no changes but after 24, 48 and 72 hours these factors showed significant differences between two groups. There were no significant differences between bilirubin levels after 24 hours in both groups but there were after 48 and 72 hours. There were no significant differences in levels of nitrogen urea during 72 hours. Considering to significant changes showed by statistical analysis of biochemical factors which results in two groups after study and also due to no significant changes in clinical condition, We suggest more study about effects of CA is necessary

[Study on effects of oral administrating of silybum marianum [L.] gaertn. extract [silymarin] on biochemical factors and tissue changes caused by aflatoxin in broiler chickens]

In poultry industry the most common and important mycotoxin is aflatoxin, and decreasing its economical and sanitary damages by using effective and inexpensive substances is the target of most of the researchers. Since the pathologic effects of aflatoxin toxicity are more important in liver, kidney and muscles, and due to the mechanism of formation of the damages and also taking in to account the other studies on silymarin effects [extract of Silybum marianum] in preventing various liver and kidney diseases, the silymarin effects reviewed on preventing the losses caused by aflatoxin due to the poultry breeding farms conditions in Iran. In this study 56 one-day chicken, divided in to 4 groups, each group contains 14 chickens. First group were feed with normal allotment, second group with normal allotment with added 1 mg/Kg aflatoxin, third group with normal allotment with 800 mg silymarin per kg body weight and with added 1 mg/Kg aflatoxin, and after 42 days biochemical, autopsy and histopathology surveys have been done on the samples. According to the reviewed data and comparing the results of studied groups, silymarin can be recommended for decreasing the damages caused by aflatoxin during the period of breeding broiler chickens

Study on effects of topical use of Silybum marianum extract [silymarin] on U.V. irradiated guinea pig skin

Regarding to the skin damages caused by the ultraviolet ray of sun and the need for protective materials against these mal effects, Silymarin was evaluated for the porpose. In this study, by histopathology studies and surveying the clinical symptoms the external use of an herbaceous [herbal] substance, Silymarin, in protecting the mal effects of ultraviolet ray has been evaluated. For the study 60 albino hamsters with same age and gender has been selected and they divided in two groups [each group 30 hamsters] randomly. The hair on the back of all animals shaved in an area about 2 square centimeters. The first group gets 9 miligram Silymarin with 20 micro liter acetone topically, and the second group gets only 20 micro liter acetone topically. Then both groups for 45 days exposed to ultra violet ray, 180 milijoule/cm[2] each day. The results of clinical and histopathologic surveys show that topical usage of Silymarin has considerable protective effects against the mal effects of ultra violet ray on skin and this can be a promise for using this herbaceous substance as a topical sun protects substance